《植物生理学报》 2012, 48(12): 1193-1199

H₂S位于H₂O₂下游参与乙烯诱导拟南芥气孔关闭过程

侯智慧, 车永梅, 王兰香, 侯丽霞, 刘新*

青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109

通信作者：刘新；E-mail: liuxin6080@yahoo.com.cn；Tel: 0532-88030224

摘　要：

H2O2和H2S是植物体内重要的信号分子, 二者均参与乙烯诱导的拟南芥气孔关闭过程。以拟南芥野生型及其突变体 为材料研究了H2O2和H2S在乙烯诱导拟南芥气孔关闭过程中的相互关系。结果表明, 乙烯能够诱导野生型拟南芥叶片H2S 含量及L-/D-半胱氨酸脱巯基酶(L-/D-CDes)活性显著增加, 促进气孔关闭, 但对H2O2合成突变体AtrbohD、AtrbohF、Atpao2 和Atpao4植株叶片无显著作用; 乙烯亦可引起H2S合成突变体Atl-cdes和Atd-cdes气孔保卫细胞H2O2水平的显著增加, 但对其 气孔运动没有显著作用。此外, H2O2清除剂和合成抑制剂均能抑制乙烯诱导的拟南芥叶片H2S含量和L-/D-CDes活性的增 加及气孔开度的减小; 而H2S清除剂和合成抑制剂虽能抑制乙烯诱导的气孔关闭, 却不能改变乙烯对拟南芥叶片气孔保卫 细胞H2O2的作用效应。由此表明H2S位于H2O2下游介导乙烯诱导拟南芥气孔关闭过程。

关键词：硫化氢; 过氧化氢; 乙烯; 气孔关闭

收稿：2012-08-13   修定：2012-10-17

资助：国家自然科学基金(30970228和31170237)、山东省自然科 学基金(ZR2010CM024)和植物生理学与生物化学国家重 点实验室开放课题(SKLPPBKF11001)。

H₂S Functions Downstream of H₂O₂ in Mediating Ethylene-Induced Stomatal Closure in Arabidopsis thaliana

HOU Zhi-Hui, CHE Yong-Mei, WANG Lan-Xiang, HOU Li-Xia, LIU Xin*

Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong 266109, China

Corresponding author: LIU Xin; E-mail: liuxin6080@yahoo.com.cn; Tel: 0532-88030224

Abstract:

Hydrogen sulfide (H₂S) and hydrogen peroxide (H₂O₂) as important plant signaling molecules both participate in ethylene induced stomatal closure in Arabidopsis thaliana, but the relationship between them is unclear. So we used A. thaliana wide type and related mutants as materials to study the interaction between H₂S and H₂O₂ in mediating ethylene induced stomatal closure. The results showed that ethylene triggered raise of H₂S content and L-/D-cysteine desulfhydrase (L-/D-CDes) activity as well as stomatal closure in leaves of A. thaliana wild type, but not in those of H₂O₂ deficient mutants AtrbohD, AtrbohF, Atpao2 and Atpao4, ethylene induced increase in H₂O₂ content in H₂S deficient mutants Atl-cdes and Atd-cdes, but couldn’t induce stomatal closure. H₂O₂ scavenger and synthesis inhibitors depressed the inducing effect of ethylene on H₂S content, L-/D-CDes activity as well as stomatal closure in leaves of A. thaliana. However, although H₂S scavenger and synthesis inhibitors suppressed ethylene-induced stomatal closure, but had no significant effect on ethyleneinduced increase in H₂O₂ content in guard cells of A. thaliana. From these data it can be deduced that H₂S acts downstream of H₂O₂ mediating ethylene-induce stomatal closure in A. thaliana.

Key words: hydrogen sulfide; hydrogen peroxide; ethylene; stomatal closure